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Human Phospholipase A2, Group III (PLA2G3) ELISA Kit

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Human Phospholipase A2, Group III (PLA2G3) ELISA Kit

Place of Origin : China , Canada
Brand Name : DLdevelop
Certification : CE, ISO9001:2015
MOQ : 48T
Price : Negotiation
Packaging Details : Carton Box
Delivery Time : 1-2 Working Days
Payment Terms : TT
Supply Ability : Abundant Supply
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Human Phospholipase A2, Group III (PLA2G3) ELISA Kit


two product lines: Traditional ELISA Kit and Ready-to-Use ELISA Kit.

Traditional ELISA KitReady-to-Use ELISA KIT
Product name:Human Phospholipase A2, Group III (PLA2G3) ELISA Kit

GIII-SPLA2; sPLA2-III; Group 3 secretory phospholipase A2; Group III secretory phospholipase A2; Phosphatidylcholine 2-acylhydrolase 3

Detection range:0.781-50ng/mL
Quality guarantee period:for 12 months
Catalog number:DL-PLA2G3-Hu (traditional)DLR-PLA2G3-Hu (ready-to-use)
Assay length1-4.5Hours1-3.5Hours
  • Competitive price.
  • High sensitivity.
  • High stability.
  • 12 months shelf life.
  • Pre-diluted Detection Reagent A and B
  • Reduction in the number of steps when conducting the test
  • All the reagents can be stored at 4℃
  • Faster reaction compare to other brands
  • 12 months shelf life
Instruction Manual

The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of PLA2G3 in human serum, plasma or other biological fluids.

CompositionTraditional ELISA KitReady-to-Use ELISA KITConform
Pre-coated, ready to use 96-well strip plate 1Pre-coated, ready to use 96-well strip plate 1
Plate sealer for 96 wells 2Plate sealer for 96 wells 2
Standard 2Standard 2
Diluents buffer 1×45mLStandard Diluent 1×20mL
Detection Reagent A 1×120LDetection Solution A 1×12mL
Detection Reagent B 1×120LDetection Solution B 1×12mL
TMB Substrate 1×9mLTMB Substrate 1×9mL
Stop Solution 1×6mLStop Solution 1×6mL
Wash Buffer (30concentrate) 1×20mLWash Buffer (30concentrate) 1×20mL
Instruction manual 1Instruction manual 1

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to PLA2G3. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to PLA2G3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated.

After TMB substrate solution is added, only those wells that contain PLA2G3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm10nm.

The concentration of PLA2G3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.


Matrices listed below were spiked with certain level of recombinant PLA2G3 and the recovery rates were calculated by comparing the measured value to the expected amount of PLA2G3 in samples.

MatrixRecovery range (%)Average(%)
EDTA plasma(n=5)85-10394
heparin plasma(n=5)87-9993


The linearity of the kit was assayed by testing samples spiked with appropriate concentration of PLA2G3 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.


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