Human Phospholipase A2, Group III (PLA2G3) ELISA Kit
two product lines: Traditional ELISA Kit and Ready-to-Use ELISA
|Traditional ELISA Kit||Ready-to-Use ELISA KIT|
|Product name:||Human Phospholipase A2, Group III (PLA2G3) ELISA Kit|
GIII-SPLA2; sPLA2-III; Group 3 secretory phospholipase A2; Group
III secretory phospholipase A2; Phosphatidylcholine 2-acylhydrolase
|Quality guarantee period:||for 12 months|
|Catalog number:||DL-PLA2G3-Hu (traditional)||DLR-PLA2G3-Hu (ready-to-use)|
- Competitive price.
- High sensitivity.
- High stability.
- 12 months shelf life.
- Pre-diluted Detection Reagent A and B
- Reduction in the number of steps when conducting the test
- All the reagents can be stored at 4℃
- Faster reaction compare to other brands
- 12 months shelf life
The kit is a sandwich enzyme immunoassay for the in vitro
quantitative measurement of PLA2G3 in human serum, plasma or other
|Composition||Traditional ELISA Kit||Ready-to-Use ELISA KIT||Conform|
|Pre-coated, ready to use 96-well strip plate 1||Pre-coated, ready to use 96-well strip plate 1|
|Plate sealer for 96 wells 2||Plate sealer for 96 wells 2|
|Standard 2||Standard 2|
|Diluents buffer 1×45mL||Standard Diluent 1×20mL|
|Detection Reagent A 1×120L||Detection Solution A 1×12mL|
|Detection Reagent B 1×120L||Detection Solution B 1×12mL|
|TMB Substrate 1×9mL||TMB Substrate 1×9mL|
|Stop Solution 1×6mL||Stop Solution 1×6mL|
|Wash Buffer (30concentrate) 1×20mL||Wash Buffer (30concentrate) 1×20mL|
|Instruction manual 1||Instruction manual 1|
The microtiter plate provided in this kit has been pre-coated with
an antibody specific to PLA2G3. Standards or samples are then added
to the appropriate microtiter plate wells with a biotin-conjugated
antibody preparation specific to PLA2G3. Next, Avidin conjugated to
Horseradish Peroxidase (HRP) is added to each microplate well and
After TMB substrate solution is added, only those wells that
contain PLA2G3, biotin-conjugated antibody and enzyme-conjugated
Avidin will exhibit a change in color. The enzyme-substrate
reaction is terminated by the addition of sulphuric acid solution
and the color change is measured spectrophotometrically at a
wavelength of 450nm10nm.
The concentration of PLA2G3 in the samples is then determined by
comparing the O.D. of the samples to the standard curve.
Matrices listed below were spiked with certain level of recombinant
PLA2G3 and the recovery rates were calculated by comparing the
measured value to the expected amount of PLA2G3 in samples.
|Matrix||Recovery range (%)||Average(%)|
The linearity of the kit was assayed by testing samples spiked with
appropriate concentration of PLA2G3 and their serial dilutions. The
results were demonstrated by the percentage of calculated
concentration to the expected.