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Wuxi Donglin Sci & Tech Development Co.,Ltd.
Wuxi Donglin Sci & Tech Development Co.,Ltd.
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Human Dual Serine/Threonine And Tyrosine Protein Kinase (DSTYK) ELISA Kit

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Human Dual Serine/Threonine And Tyrosine Protein Kinase (DSTYK) ELISA Kit

Place of Origin : China , Canada
Brand Name : DLdevelop
Certification : CE, ISO9001:2015
MOQ : 48T
Price : Negotiation
Packaging Details : Carton Box
Delivery Time : 1-2 Working Days
Payment Terms : TT
Supply Ability : Abundant Supply
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Human Dual Serine/Threonine And Tyrosine Protein Kinase (DSTYK) ELISA Kit

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two product lines: Traditional ELISA Kit and Ready-to-Use ELISA Kit.

Traditional ELISA KitReady-to-Use ELISA KIT
Product name:Human Dual Serine/Threonine And Tyrosine Protein Kinase (DSTYK) ELISA Kit
Method:Sandwich
Synonyms:

RIPK5; Dusty PK; RIP5; SgK496; Receptor Interacting Protein Kinase 5; RIP-homologous kinase; Sugen kinase 496; Receptor-interacting serine/threonine-protein kinase 5

Detection range:0.156-10ng/mL
Reactivity:DSTYK
Size:96T/48T
Quality guarantee period:for 12 months
Catalog number:DL-DSTYK-Hu (traditional)DLR-DSTYK-Hu (ready-to-use)
Assay length1-4.5Hours1-3.5Hours
Advantages:
  • Competitive price.
  • High sensitivity.
  • High stability.
  • 12 months shelf life.
  • Pre-diluted Detection Reagent A and B
  • Reduction in the number of steps when conducting the test
  • All the reagents can be stored at 4℃
  • Faster reaction compare to other brands
  • 12 months shelf life
Instruction Manual
Introduction
ItemStandardTest
Description

The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of DSTYK in human tissue homogenates or other biological fluids.

Conform
IdentificationColorimetricPositive
CompositionTraditional ELISA KitReady-to-Use ELISA KITConform
Pre-coated, ready to use 96-well strip plate 1Pre-coated, ready to use 96-well strip plate 1
Plate sealer for 96 wells 2Plate sealer for 96 wells 2
Standard 2Standard 2
Diluents buffer 1×45mLStandard Diluent 1×20mL
Detection Reagent A 1×120LDetection Solution A 1×12mL
Detection Reagent B 1×120LDetection Solution B 1×12mL
TMB Substrate 1×9mLTMB Substrate 1×9mL
Stop Solution 1×6mLStop Solution 1×6mL
Wash Buffer (30concentrate) 1×20mLWash Buffer (30concentrate) 1×20mL
Instruction manual 1Instruction manual 1

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to DSTYK. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to DSTYK. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated.

After TMB substrate solution is added, only those wells that contain DSTYK, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm10nm.

The concentration of DSTYK in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Recovery

Matrices listed below were spiked with certain level of recombinant DSTYK and the recovery rates were calculated by comparing the measured value to the expected amount of DSTYK in samples.

MatrixRecovery range (%)Average(%)
serum(n=5)82-10292
EDTA plasma(n=5)91-10095
heparin plasma(n=5)88-10094

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of DSTYK and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

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Sample1:21:4



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