Human Dual Serine/Threonine And Tyrosine Protein Kinase (DSTYK)
two product lines: Traditional ELISA Kit and Ready-to-Use ELISA
|Traditional ELISA Kit||Ready-to-Use ELISA KIT|
|Product name:||Human Dual Serine/Threonine And Tyrosine Protein Kinase (DSTYK)
RIPK5; Dusty PK; RIP5; SgK496; Receptor Interacting Protein Kinase
5; RIP-homologous kinase; Sugen kinase 496; Receptor-interacting
serine/threonine-protein kinase 5
|Quality guarantee period:||for 12 months|
|Catalog number:||DL-DSTYK-Hu (traditional)||DLR-DSTYK-Hu (ready-to-use)|
- Competitive price.
- High sensitivity.
- High stability.
- 12 months shelf life.
- Pre-diluted Detection Reagent A and B
- Reduction in the number of steps when conducting the test
- All the reagents can be stored at 4℃
- Faster reaction compare to other brands
- 12 months shelf life
The kit is a sandwich enzyme immunoassay for the in vitro
quantitative measurement of DSTYK in human tissue homogenates or
other biological fluids.
|Composition||Traditional ELISA Kit||Ready-to-Use ELISA KIT||Conform|
|Pre-coated, ready to use 96-well strip plate 1||Pre-coated, ready to use 96-well strip plate 1|
|Plate sealer for 96 wells 2||Plate sealer for 96 wells 2|
|Standard 2||Standard 2|
|Diluents buffer 1×45mL||Standard Diluent 1×20mL|
|Detection Reagent A 1×120L||Detection Solution A 1×12mL|
|Detection Reagent B 1×120L||Detection Solution B 1×12mL|
|TMB Substrate 1×9mL||TMB Substrate 1×9mL|
|Stop Solution 1×6mL||Stop Solution 1×6mL|
|Wash Buffer (30concentrate) 1×20mL||Wash Buffer (30concentrate) 1×20mL|
|Instruction manual 1||Instruction manual 1|
The microtiter plate provided in this kit has been pre-coated with
an antibody specific to DSTYK. Standards or samples are then added
to the appropriate microtiter plate wells with a biotin-conjugated
antibody preparation specific to DSTYK. Next, Avidin conjugated to
Horseradish Peroxidase (HRP) is added to each microplate well and
After TMB substrate solution is added, only those wells that
contain DSTYK, biotin-conjugated antibody and enzyme-conjugated
Avidin will exhibit a change in color. The enzyme-substrate
reaction is terminated by the addition of sulphuric acid solution
and the color change is measured spectrophotometrically at a
wavelength of 450nm10nm.
The concentration of DSTYK in the samples is then determined by
comparing the O.D. of the samples to the standard curve.
Matrices listed below were spiked with certain level of recombinant
DSTYK and the recovery rates were calculated by comparing the
measured value to the expected amount of DSTYK in samples.
|Matrix||Recovery range (%)||Average(%)|
The linearity of the kit was assayed by testing samples spiked with
appropriate concentration of DSTYK and their serial dilutions. The
results were demonstrated by the percentage of calculated
concentration to the expected.